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A revTetR provided herein has the following functional properties: 1) the polypeptide can bind to a tet operator sequence, ie, it retains the DNA binding specificity of a wild-type Tet repressor; and 2) it is regulated in a reverse manner by tetracycline than a wild-type Tet repressor, ie, the modified Tet repressor binds to a tet operator sequence with a greater binding affinity (or a lower dissociation constant, K d) in the presence of Tc or Tc analog, than in the absence of Tc or its analog?

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Hence, tetracyclines became known as "broad spectrum" antibiotics!

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